Biotechnology process and application pdf

Which of the following statements is false? Insulin was originally extracted from pancreas of slaughtered pigs and cattle II. Animal insulin is difficult to obtain III. Animal insulin is identical to human insulin IV. Non human insulin caused some patients to develop allergy V. Recombinant DMA allowed scientists to insert a human insulin gene into a bacterial expression sector VI. Recombinant insulin is actually obtained from E. Which of the following nematode infects the roots of tobacco plants and causes a great reduction in yield?

Introduction of trangenes will result in — a Formation of new species b Formation of new protein c Alter a biosynthetic pathway d Both b and c. Bt toxin is harmful to insects like — a Lepidoterans tobacco budworm, armyworms b Coleopterans bettles c Dipterans flies and mosquito d All. Bt toxin is — a Extotoxin biodegradable insecticide b Extotoxin, bionondegradable insecticide c Endotoxin biodegradable insecticide d Endotoxin, bionondegradable insecticide.

Recombinant DNAtechnology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant.

Bt cotton is resistant to bollworm infestation. Bacillus thuringiensis form cry protein during any phase of their growth IV. Bacillus thuringiensis is not harmed by self Cry protein because of its occurrence as protoxin inactive V. Which of the following techniques serve the purpose of early diagnosis? Genetically modified tobacco plant with Bt gene is resistant to — a Bollworms b Hornworms c Hookworms d Roundworms. Bt is resistant to — a Viruses b Abiotic stress c Bollworm d Herbicide.

When a patient with defective ADA was treated, which of the following steps was performed for gene therapy? Lymphocytes were obtained from the patients , II. Lymphocytes are transferred to culture dishes III.

Why is repeated transfusions of genetically engineered cells required in SCID patients? Which of the following could be a permanent cure for treatment of severe combined immunodeficiency SCID — a Bone marrow transplantation b Gene therapy c Enzyme replacement therapy d Both a and c. Which of the following is the source of complementary strand in mRNA silencing. The first clinical gene therapy was given in to a 4 years old girl with which of the following enzyme deficiency?

Which of the following is a correct statement? How does Bt toxin kill the larvae of certain insects? Why insulin not administered orally to diabetic patient? Which of the following peptide chain is removed during maturation of pro-insulin into insulin? A plant expressing a gene from another organisms is — a Transgenic b Clone c Transformed d Somoclonal variant. Which technique would you expect to be completely curative in SCI D? Share Email. Top clipped slide.

Download Now Download Download to read offline. Biotechnology: Process and Application May. Firdous Ansari Follow. Teacher at Sree Narayana Guru College. Gene transfer technologies. Genetic Engineering. Gene transformation methods. Plant transformation methods. Transformation in bacteria. Single cell protein. Heterologous proteins.

Steroid receptor. Related Books Free with a 30 day trial from Scribd. Related Audiobooks Free with a 30 day trial from Scribd. Elizabeth Howell. Biotechnology: Process and Application 1. Oldest form of biotechnology 3. Application of fermentation in production of wine and other alcoholic beverages is also a biotechnological technique 4. But with time biotechnology gradually became more sophisticated. Biotechnology led to production of many products and provides many services for human welfare.

Dragon Fly The biotechnology industry has mushroomed since , with U. There were , people employed by U. In-Vitro Fertilization Also called as Test tube baby 9.

Plant tissue culture DNA vaccines Dale Kaiser The present day rDNA technology flourished after the work of Gene coding for Plasmid antibiotic resistance Vector Cloning Salmonella typhimurium They successfully linked a gene coding for antibiotic resistance with a native plasmid of Salmonella typhimurium with the vector plasmid and then cloning it in E. What is recombinant DNA? Seedless guava Calorie free sugar Introducing the recombinant Introducing vector into a competent host in Host cell Culturing these cells to obtain Culturing multiple copies or clones of the cells desired DNA fragments Using these copies to transform suitable host cells Transformation so as to express the desired of host cell gene.

Example: Production of Insulin Enzyme Transposase Cuts out transposon Ligates in new position Joshua Lederberg Plasmid Eg. Bands Construction of Genomic Library. Entire genome isolated Cut into fragments by REN Fragments inserted in Vector Recombinant vectors are transferred into suitable organism Transferred organisms are cultured and stored Reverse Transcriptase.

PCR Machine Steps of PCR technique The double strand melts open to single stranded DNA, all enzymatic reactions stop for example : the extension from a previous cycle.

Ionic bonds are constantly formed and broken between the single stranded primer and the single stranded template. Once there are a few bases built in, the ionic bond is so strong between the template and the primer, that it does not break anymore.

The bases complementary to the template are coupled to the primer on the 3' side the polymerase adds dNTP's from 5' to 3', reading the template from 3' to 5' side, bases are added complementary to the template The exponential amplification of the gene in PCR. Application of biotechnology in agriculture- Bt crops Crop plants are now engineered to express Bt toxin. Bt crops are now commercially available. For Eg. Tumor Ti Plasmid Nif Gene isolated from Rhizobium